http://hdl.handle.net/20.500.12701/1816 Parasites & Vectors is an open access, peer-reviewed online journal dealing with the biology of parasites, parasitic diseases, intermediate hosts, vectors and vector-borne pathogens. Fri, 03 May 2024 12:28:29 GMT 2024-05-03T12:28:29Z http://hdl.handle.net/20.500.12701/1817 Название: Hemozoin “knobs” in Opisthorchis felineus infected liver Авторы: Pershina, Alexandra G.; Saltykova, Irina V.; Ivanov, Vladimir V.; Perina, Ekaterina A.; Demin, Alexander M.; Shevelev, Oleg B.; Buzueva, Irina I.; Gutakovskii, Anton K.; Vtorushin, Sergey V.; Ganebnykh, Ilya N.; Krasnov, Victor P.; Sazonov, Alexey E.; Ogorodova, Ludmila M. Краткий осмотр (реферат): Background Hemozoin is the pigment produced by some blood-feeding parasites. It demonstrates high diagnostic and therapeutic potential. In this work the formation of co-called hemozoin “knobs” – the bile duct ectasia filled up by hemozoin pigment - in Opisthorhis felineus infected hamster liver has been observed. Methods The O. felineus infected liver was examined by histological analysis and magnetic resonance imaging (MRI). The pigment hemozoin was identified by Fourier transform infrared spectroscopy and high resolution electrospray ionization mass spectrometry analysis. Hemozoin crystals were characterised by high resolution transmission electron microscopy. Results Hemozoin crystals produced by O. felineus have average length 403 nm and the length-to-width ratio equals 2.0. The regurgitation of hemozoin from parasitic fluke during infection leads to formation of bile duct ectasia. The active release of hemozoin from O. felineus during in vitro incubation has also been evidenced. It has been shown that the hemozoin knobs can be detected by magnetic resonance imaging. Conclusions In the paper for the first time the characterisation of hemozoin pigment extracted from liver fluke O. felineus has been conducted. The role of hemozoin in the modification of immune response by opisthorchiasis is assumed. Thu, 17 Sep 2015 00:00:00 GMT http://hdl.handle.net/20.500.12701/1817 2015-09-17T00:00:00Z