DSpace Собрание: Molecules is the leading international, peer-reviewed, open access journal of chemistry.
http://hdl.handle.net/20.500.12701/1642
Molecules is the leading international, peer-reviewed, open access journal of chemistry.2024-02-22T13:48:42ZRadionuclide Molecular Imaging of EpCAM Expression in Triple-Negative Breast Cancer Using the Scaffold Protein DARPin Ec1
http://hdl.handle.net/20.500.12701/1975
Название: Radionuclide Molecular Imaging of EpCAM Expression in Triple-Negative Breast Cancer Using the Scaffold Protein DARPin Ec1
Авторы: Vorobyeva, Anzhelika; Bezverkhniaia, Ekaterina; Konovalova, Elena; Schulga, Alexey; Garousi, Javad; Vorontsova, Olga; Abouzayed, Ayman; Orlova, Anna; Deyev, Sergey; Tolmachev, Vladimir
Краткий осмотр (реферат): Efficient treatment of disseminated triple-negative breast cancer (TNBC) remains an unmet clinical need. The epithelial cell adhesion molecule (EpCAM) is often overexpressed on the surface of TNBC cells, which makes EpCAM a potential therapeutic target. Radionuclide molecular imaging of EpCAM expression might permit selection of patients for EpCAM-targeting therapies. In this study, we evaluated a scaffold protein, designed ankyrin repeat protein (DARPin) Ec1, for imaging of EpCAM in TNBC. DARPin Ec1 was labeled with a non-residualizing [125I]I-para-iodobenzoate (PIB) label and a residualizing [99mTc]Tc(CO)3 label. Both imaging probes retained high binding specificity and affinity to EpCAM-expressing MDA-MB-468 TNBC cells after labeling. Internalization studies showed that Ec1 was retained on the surface of MDA-MB-468 cells to a high degree up to 24 h. Biodistribution in Balb/c nu/nu mice bearing MDA-MB-468 xenografts demonstrated specific uptake of both [125I]I-PIB-Ec1 and [99mTc]Tc(CO)3-Ec1 in TNBC tumors. [125I]I-PIB-Ec1 had appreciably lower uptake in normal organs compared with [99mTc]Tc(CO)3-Ec1, which resulted in significantly (p < 0.05) higher tumor-to-organ ratios. The biodistribution data were confirmed by micro-Single-Photon Emission Computed Tomography/Computed Tomography (microSPECT/CT) imaging. In conclusion, an indirectly radioiodinated Ec1 is the preferable probe for imaging of EpCAM in TNBC.2020-10-14T00:00:00ZNa+i,K+i-Dependent and -Independent Signaling Triggered by Cardiotonic Steroids: Facts and Artifacts
http://hdl.handle.net/20.500.12701/1759
Название: Na+i,K+i-Dependent and -Independent Signaling Triggered by Cardiotonic Steroids: Facts and Artifacts
Авторы: Orlov, Sergei N.; Klimanova, Elizaveta A.; Tverskoi, Artem M.; Vladychenskaya, Elizaveta A.; Smolyaninova, Larisa V.; Lopina, Olga D.
Краткий осмотр (реферат): Na+,K+-ATPase is the only known receptor of cardiotonic steroids (CTS) whose interaction with catalytic α-subunits leads to inhibition of this enzyme. As predicted, CTS affect numerous cellular functions related to the maintenance of the transmembrane gradient of monovalent cations, such as electrical membrane potential, cell volume, transepithelial movement of salt and osmotically-obliged water, symport of Na+ with inorganic phosphate, glucose, amino acids, nucleotides, etc. During the last two decades, it was shown that side-by-side with these canonical Na+i/K+i-dependent cellular responses, long-term exposure to CTS affects transcription, translation, tight junction, cell adhesion and exhibits tissue-specific impact on cell survival and death. It was also shown that CTS trigger diverse signaling cascades via conformational transitions of the Na+,K+-ATPase α-subunit that, in turn, results in the activation of membrane-associated non-receptor tyrosine kinase Src, phosphatidylinositol 3-kinase and the inositol 1,4,5-triphosphate receptor. These findings allowed researchers to propose that endogenous CTS might be considered as a novel class of steroid hormones. We focus our review on the analysis of the relative impact Na+i,K+i-mediated and -independent pathways in cellular responses evoked by CTS.2017-04-14T00:00:00ZHeterogeneity of Circulating Tumor Cells in Neoadjuvant Chemotherapy of Breast Cancer
http://hdl.handle.net/20.500.12701/1735
Название: Heterogeneity of Circulating Tumor Cells in Neoadjuvant Chemotherapy of Breast Cancer
Авторы: Kaigorodova, Evgeniya V.; Savelieva, Olga E.; Tashireva, Liubov A.; Tarabanovskaya, Natalia A.; Simolina, Elena I.; Denisov, Evgeny V.; Slonimskaya, Elena M.; Choynzonov, Evgeny L.; Perelmuter, Vladimir M.
Краткий осмотр (реферат): The biological properties of circulating tumor cells (CTCs), and their dynamics during neoadjuvant chemotherapy are important, both for disease progression prediction and therapeutic target determination, with the aim of preventing disease progression. The aim of our study was to estimate of different CTC subsets in breast cancer during the NACT (neoadjuvant chemotherapy). The prospective study includes 27 patients with invasive breast cancer, T2-4N0-3M0, aged 32 to 60 years. Venous heparinized blood samples, taken before and after biopsy, after each courses of chemotherapy (on days 3–7), and before surgical intervention, served as the material for this study. Different subsets of circulating tumor cells were determined on the basis of the expression of EpCAM, CD45, CD44, CD24, and N-Cadherin using flow cytometry. As the result of this study, it has been observed that significant changes in the quantity of the different subsets of circulating tumor cells in patients’ blood were observed after carrying out the 3rd course of NACT. NACT causes significant changes in the quantity of six CTC subsets, with various combinations of stemness and epithelial-mesenchymal transition (EMT) properties.2018-03-22T00:00:00ZPhysicochemical Characterization and Antioxidant Activity of Humic Acids Isolated from Peat of Various Origins
http://hdl.handle.net/20.500.12701/1731
Название: Physicochemical Characterization and Antioxidant Activity of Humic Acids Isolated from Peat of Various Origins
Авторы: Zykova, Maria V.; Schepetkin, Igor A.; Belousov, Mikhail V.; Krivoshchekov, Sergey V.; Logvinova, Lyudmila A.; Bratishko, Kristina A.; Yusubov, Mekhman S.; Romanenko, Sergey V.; Quinn, Mark T.
Краткий осмотр (реферат): Although humic acids (HAs) from peat exhibit various therapeutic properties, there is little information available concerning their physicochemical and antioxidant properties. To address this issue, nine different types of peat, including oligotrophic, mesotrophic, and minerotrophic peat samples, were used for isolation of HA fractions by basic (HAb) and pyrophosphate (HAp) extractions. Physical parameters of the HAs were analyzed by UV-Vis, fluorescent, infrared (IR), and electron paramagnetic resonance (EPR) spectroscopy. Average Mr of the fractions ranged from 17.2 to 39.7 kDa, while their humification index (HIX) varied from 0.49 to 1.21. HAp fractions had a higher content of aromatic structures compared to HAb fractions. Moreover, HAp fractions had a significantly higher content of phenolic OH groups (3.6 ± 0.5 mmol/g) versus HAb (3.1 ± 0.5 mmol/g). All HA fractions exhibited antioxidant activity in radical scavenging and electrochemical assays, and their EPR signal had a single line with g = 2.0035, which is consistent with semiquinone type radicals. Furthermore, the HIX was found to be important in determining the number of semiquinone-type free radicals in the HA structures. Overall, these data provide a molecular basis to explain at least part of the beneficial therapeutic properties of peat-derived HAs.2018-03-24T00:00:00Z