DSpace Собрание: IJMS
http://hdl.handle.net/20.500.12701/1631
IJMS2024-02-22T16:58:38ZAntidiabetic Effects of Bisamide Derivative of Dicarboxylic Acid in Metabolic Disorders
http://hdl.handle.net/20.500.12701/2048
Название: Antidiabetic Effects of Bisamide Derivative of Dicarboxylic Acid in Metabolic Disorders
Авторы: Pakhomova, Angelina Vladimirovn; Nebolsin, Vladimir Evgenievich; Pershina, Olga Victorovna; Krupin, Vyacheslav Andreevich; Sandrikina, Lubov Alexandrovna; Pan, Edgar Sergeevich; Ermakova, Natalia Nicolaevna; Vaizova, Olga Evgenevna; Widera, Darius; Grimm, Wolf-Dieter; Kravtsov, Viacheslav Yur’evich; Afanasiev, Sergey Alexandrovich; Morozov, Sergey Georgievich; Kubatiev, Aslan Amirkhanovich; Dygai, Alexander Mikhaylovich; Skurikhin, Evgenii Germanovich
Краткий осмотр (реферат): In clinical practice, the metabolic syndrome can lead to multiple complications, including diabetes. It remains unclear which component of the metabolic syndrome (obesity, inflammation, hyperglycemia, or insulin resistance) has the strongest inhibitory effect on stem cells involved in beta cell regeneration. This makes it challenging to develop effective treatment options for complications such as diabetes. In our study, experiments were performed on male C57BL/6 mice where metabolic disorders have been introduced experimentally by a combination of streptozotocin-treatment and a high-fat diet. We evaluated the biological effects of Bisamide Derivative of Dicarboxylic Acid (BDDA) and its impact on pancreatic stem cells in vivo. To assess the impact of BDDA, we applied a combination of histological and biochemical methods along with a cytometric analysis of stem cell and progenitor cell markers. We show that in mice with metabolic disorders, BDDA has a positive effect on lipid and glucose metabolism. The pancreatic restoration was associated with a decrease of the inhibitory effects of inflammation and obesity factors on pancreatic stem cells. Our data shows that BDDA increases the number of pancreatic stem cells. Thus, BDDA could be used as a new compound for treating complication of the metabolic syndrome such as diabetes.2020-02-03T00:00:00ZHeterogeneity of Stemlike Circulating Tumor Cells in Invasive Breast Cancer
http://hdl.handle.net/20.500.12701/2037
Название: Heterogeneity of Stemlike Circulating Tumor Cells in Invasive Breast Cancer
Авторы: Savelieva, Olga E.; Tashireva, Liubov A.; Kaigorodova, Evgeniya V.; Buzenkova, Angelina V.; Mukhamedzhanov, Rustam Kh.; Grigoryeva, Evgeniya S.; Zavyalova, Marina V.; Tarabanovskaya, Natalia A.; Cherdyntseva, Nadezhda V.; Perelmuter, Vladimir M.
Краткий осмотр (реферат): The presence of stem and epithelial–mesenchymal-transition (EMT) features in circulating tumor cells (CTCs) determines their invasiveness, adaptability to the microenvironment, and resistance to proapoptotic signals and chemotherapy. It also allows them to fulfil the role of metastatic “seeds”. We evaluated the heterogeneity of stem CTCs by their CD44, ALDH1, and CD133 expression depending on N-cadherin expression in breast-cancer patients. A total of 38 female patients were selected for this study. CTC phenotypes were determined by flow cytometry before any type of treatment. Multiplex immunofluorescence was used for the evaluation of tumor-cell heterogeneity in primary lesions. In patients who had CD44-CD24- CTCs, a subset of cells with the expression of other stem-cell markers (CD133 and ALDH1) were detected. Expression of CD133 and/or ALDH1 may be associated with expression of N-cadherin: all populations of N-cadherin+ CTCs demonstrate stem features; in the absence of N-cadherin expression, true nonstem (CD44-CD24-CD133-ALDH1-) cells are found. The heterogeneity of stem marker expression in CTCs was observed regardless of N-cadherin expression. In our study, stromal cell-derived factor-1 (SDF-1) receptor expression in CTCs did not depend on stemlike traits, but was instead associated with N-cadherin expression. Subpopulations of tumor cells, detected both in tumors and blood, were identified. Breast cancer was characterized by pronounced interpersonal and intrapersonal heterogeneity of CTCs by the presence and combination of various stem features and N-cadherin expression. To complete the characterization of stemlike features of CTCs, we suggest the simultaneous use of the three stem markers.2020-04-16T00:00:00ZInfluence of Hypoxic and Hyperoxic Preconditioning on Endothelial Function in a Model of Myocardial Ischemia-Reperfusion Injury with Cardiopulmonary Bypass (Experimental Study)
http://hdl.handle.net/20.500.12701/2024
Название: Influence of Hypoxic and Hyperoxic Preconditioning on Endothelial Function in a Model of Myocardial Ischemia-Reperfusion Injury with Cardiopulmonary Bypass (Experimental Study)
Авторы: Mandel, Irina A.; Podoksenov, Yuri K.; Suhodolo, Irina V.; An, Darya A.; Mikheev, Sergey L.; Podoksenov, Andrey Yu.; Svirko, Yulia S.; Gusakova, Anna M.; Shipulin, Vladimir M.; Yavorovskiy, Andrey G.
Краткий осмотр (реферат): The aim of the experiment was to evaluate the effect of preconditioning based on changes in inspiratory oxygen fraction on endothelial function in the model of ischemia-reperfusion injury of the myocardium in the condition of cardiopulmonary bypass. The prospective randomized study included 32 rabbits divided into four groups: hypoxic preconditioning, hyperoxic preconditioning, hypoxic-hyperoxic preconditioning, and control group. All animals were anesthetized and mechanically ventilated. We provided preconditioning, then started cardiopulmonary bypass, followed by induced acute myocardial infarction (ischemia 45 min, reperfusion 120 min). We investigated endothelin-1, nitric oxide metabolites, asymmetric dimethylarginine during cardiopulmonary bypass: before ischemia, after ischemia, and after reperfusion. We performed light microscopy of myocardium, kidney, lungs, and gut mucosa. The endothelin-1 level was much higher in the control group than in all preconditioning groups after ischemia. The endothelin-1 even further increased after reperfusion. The total concentration of nitric oxide metabolites was significantly higher after all types of preconditioning compared with the control group. The light microscopy of the myocardium and other organs revealed a diminished damage extent in the hypoxic-hyperoxic preconditioning group as compared to the control group. Hypoxic-hyperoxic preconditioning helps to maintain the balance of nitric oxide metabolites, reduces endothelin-1 hyperproduction, and enforces organ protection.2020-07-27T00:00:00ZTotal Blood Exosomes in Breast Cancer: Potential Role in Crucial Steps of Tumorigenesis
http://hdl.handle.net/20.500.12701/1976
Название: Total Blood Exosomes in Breast Cancer: Potential Role in Crucial Steps of Tumorigenesis
Авторы: Konoshenko, Maria; Sagaradze, Georgy; Orlova, Evgeniya; Shtam, Tatiana; Proskura, Ksenia; Kamyshinsky, Roman; Yunusova, Natalia; Alexandrova, Antonina; Efimenko, Anastasia; Tamkovich, Svetlana
Краткий осмотр (реферат): Exosomes are crucial players in cell-to-cell communication and are involved in tumorigenesis. There are two fractions of blood circulating exosomes: free and cell-surface-associated. Here, we compared the effect of total blood exosomes (contain plasma exosomes and blood cell-surface-associated exosomes) and plasma exosomes from breast cancer patients (BCPs, n = 43) and healthy females (HFs, n = 35) on crucial steps of tumor progression. Exosomes were isolated by ultrafiltration, followed by ultracentrifugation, and characterized by cryo-electron microscopy (cryo-EM), nanoparticle tracking analysis, and flow cytometry. Cryo-EM revealed a wider spectrum of exosome morphology with lipid bilayers and vesicular internal structures in the HF total blood in comparison with plasma. No differences in the morphology of both exosomes fractions were detected in BCP blood. The plasma exosomes and total blood exosomes of BCPs had different expression levels of tumor-associated miR-92a and miR-25-3p, induced angiogenesis and epithelial-to-mesenchymal transition (EMT), and increased the number of migrating pseudo-normal breast cells and the total migration path length of cancer cells. The multidirectional effects of HF total blood exosomes on tumor dissemination were revealed; they suppress the angiogenesis and total migration path length of MCF10A, but stimulate EMT and increase the number of migrating MCF10A and the total path length of SKBR3 cells. In addition, HF plasma exosomes enhance the metastasis-promoting properties of SKBR3 cells and stimulate angiogenesis. Both cell-free and blood cell-surface-associated exosomes are involved in the crucial stages of carcinogenesis: the initiation of EMT and the stimulation of proliferation, cell migration, and angiogenesis. Thus, for the estimation of the diagnostic/prognostic significance of circulating exosomes in the blood of cancer patients more correctly, the total blood exosomes, which consist of plasma exosomes and blood cell-surface-associated exosomes should be used.2020-10-05T00:00:00Z