PLOS Neglected Tropical Diseases is dedicated to research that addresses the neglected, the forgotten, and the under-resourced, in order to improve the health and prosperity of all the world’s people. We work alongside researchers spanning every continent who are deeply rooted in under-resourced communities. We amplify their voices through Open Access and Open Science practices to make this knowledge accessible and inspire greater change far beyond immediate borders. http://hdl.handle.net/20.500.12701/1746 2024-02-17T19:18:38Z 2024-02-17T19:18:38Z Kokova, Daria Verhoeven, Aswin Perina, Ekaterina A. Ivanov, Vladimir V. Knyazeva, Elena M. Saltykova, Irina V. Mayboroda, Oleg A. http://hdl.handle.net/20.500.12701/2057 2022-05-17T07:04:24Z 2020-01-24T00:00:00Z

Название: Plasma metabolomics of the time resolved response to Opisthorchis felineus infection in an animal model (golden hamster, Mesocricetus auratus) Авторы: Kokova, Daria; Verhoeven, Aswin; Perina, Ekaterina A.; Ivanov, Vladimir V.; Knyazeva, Elena M.; Saltykova, Irina V.; Mayboroda, Oleg A. Краткий осмотр (реферат): Background Opisthorchiasis is a hepatobiliary disease caused by flukes of the trematode family Opisthorchiidae. Opisthorchiasis can lead to severe hepatobiliary morbidity and is classified as a carcinogenic agent. Here we investigate the time-resolved metabolic response to Opisthorchis felineus infection in an animal model. Methodology Thirty golden hamsters were divided in three groups: severe infection (50 metacercariae/hamster), mild infection (15 metacercariae/hamster) and uninfected (vehicle-PBS) groups. Each group consisted of equal number of male and female animals. Plasma samples were collected one day before the infection and then every two weeks up to week 22 after infection. The samples were subjected to 1H Nuclear Magnetic Resonance (NMR) spectroscopy and multivariate statistical modelling. Principal findings The time-resolved study of the metabolic response to Opisthorchis infection in plasma in the main lines agrees with our previous report on urine data. The response reaches its peak around the 4th week of infection and stabilizes after the 10th week. Yet, unlike the urinary data there is no strong effect of the gender in the data and the intensity of infection is presented in the first two principal components of the PCA model. The main trends of the metabolic response to the infection in blood plasma are the transient depletion of essential amino acids and an increase in lipoprotein and cholesterol concentrations. Conclusions The time resolved metabolic signature of Opisthorchis infection in the hamster’s plasma shows a coherent shift in amino acids and lipid metabolism. Our work provides insight into the metabolic basis of the host response on the helminth infection.

2020-01-24T00:00:00Z Fedorova, Olga S. Fedotova, Marina M. Zvonareva, Olga I. Mazeina, Sofia V. Kovshirina, Yulia V. Sokolova, Tatiana S. Golovach, Ekaterina A. Kovshirina, Anna E. Konovalova, Uliana V. Kolomeets, Ivan L. Gutor, Sergey S. Petrov, Vyacheslav A. Hattendorf, Jan Ogorodova, Ludmila M. Odermatt, Peter http://hdl.handle.net/20.500.12701/2030 2022-05-12T05:59:13Z 2020-06-29T00:00:00Z

Название: Opisthorchis felineus infection, risks, and morbidity in rural Western Siberia, Russian Federation Авторы: Fedorova, Olga S.; Fedotova, Marina M.; Zvonareva, Olga I.; Mazeina, Sofia V.; Kovshirina, Yulia V.; Sokolova, Tatiana S.; Golovach, Ekaterina A.; Kovshirina, Anna E.; Konovalova, Uliana V.; Kolomeets, Ivan L.; Gutor, Sergey S.; Petrov, Vyacheslav A.; Hattendorf, Jan; Ogorodova, Ludmila M.; Odermatt, Peter Краткий осмотр (реферат): The liver fluke, Opisthorchis felineus, is widely distributed throughout Europe and large parts of the Russian Federation. In Western Siberia, information about opisthorchiasis is lacking although infection may lead to severe liver and bile duct diseases. We aimed to assess the current prevalence of O. felineus infection along with associated risk factors and morbidity in rural Western Siberia.

2020-06-29T00:00:00Z Ogorodova, Ludmila M. Fedorova, Olga S. Sripa, Banchob Mordvinov, Viatcheslav A. Katokhin, Aleksei V. Keiser, Jennifer Odermatt, Peter Brindley, Paul J. Mayboroda, Oleg A. Velavan, Thirumalaisamy P. Freidin, Maxim B. Sazonov, Alexey E. Saltykova, Irina V. Pakharukova, Mariya Y. Kovshirina, Yulia V. Kaloulis, Kostas Krylova, Olga Y. Yazdanbakhsh, Maria http://hdl.handle.net/20.500.12701/1821 2022-04-04T05:45:57Z 2015-04-02T00:00:00Z

Название: Opisthorchiasis: An Overlooked Danger Авторы: Ogorodova, Ludmila M.; Fedorova, Olga S.; Sripa, Banchob; Mordvinov, Viatcheslav A.; Katokhin, Aleksei V.; Keiser, Jennifer; Odermatt, Peter; Brindley, Paul J.; Mayboroda, Oleg A.; Velavan, Thirumalaisamy P.; Freidin, Maxim B.; Sazonov, Alexey E.; Saltykova, Irina V.; Pakharukova, Mariya Y.; Kovshirina, Yulia V.; Kaloulis, Kostas; Krylova, Olga Y.; Yazdanbakhsh, Maria Краткий осмотр (реферат): A group of helminth infections, caused by liver flukes of the trematode family Opisthorchiidae, were recently the focus of discussions at a meeting where scientists from Russia, Southeast Asia, Europe, and the United States came together in Tomsk city in Western Siberia (Russia) to form a Tomsk OPIsthorchiasis Consortium (TOPIC). This initiative starts a platform to raise awareness, to strengthen integrated control, and to conduct research on a neglected infectious disease that afflicts populations not only in the tropical regions of East Asia but also in temperate and semi-arctic areas of Europe and Asia ...

2015-04-02T00:00:00Z Saltykova, Irina V. Petrov, Vjacheslav A. Logacheva, Maria D. Ivanova, Polina G. Merzlikin, Nikolay V. Sazonov, Alexey E. Ogorodova, Ludmila M. Brindley, Paul J. http://hdl.handle.net/20.500.12701/1774 2022-03-31T09:24:35Z 2016-07-22T00:00:00Z

Название: Biliary Microbiota, Gallstone Disease and Infection with Opisthorchis felineus Авторы: Saltykova, Irina V.; Petrov, Vjacheslav A.; Logacheva, Maria D.; Ivanova, Polina G.; Merzlikin, Nikolay V.; Sazonov, Alexey E.; Ogorodova, Ludmila M.; Brindley, Paul J. Краткий осмотр (реферат): Background There is increasing interest in the microbiome of the hepatobiliary system. This study investigated the influence of infection with the fish-borne liver fluke, Opisthorchis felineus on the biliary microbiome of residents of the Tomsk region of western Siberia. Methodology/Principal Findings Samples of bile were provided by 56 study participants, half of who were infected with O. felineus, and all of who were diagnosed with gallstone disease. The microbiota of the bile was investigated using high throughput, Illumina-based sequencing targeting the prokaryotic 16S rRNA gene. About 2,797, discrete phylotypes of prokaryotes were detected. At the level of phylum, bile from participants with opisthorchiasis showed greater numbers of Synergistetes, Spirochaetes, Planctomycetes, TM7 and Verrucomicrobia. Numbers of > 20 phylotypes differed in bile of the O. felineus-infected compared to non-infected participants, including presence of species of the genera Mycoplana, Cellulosimicrobium, Microlunatus and Phycicoccus, and the Archaeans genus, Halogeometricum, and increased numbers of Selenomonas, Bacteroides, Rothia, Leptotrichia, Lactobacillus, Treponema and Klebsiella. Conclusions/Significance Overall, infection with the liver fluke O. felineus modified the biliary microbiome, increasing abundance of bacterial and archaeal phylotypes.

2016-07-22T00:00:00Z