BMC Genomics is an open access, peer-reviewed journal that considers articles on all aspects of genetics, genomics and proteomics. http://hdl.handle.net/20.500.12701/1688 2024-02-17T21:15:47Z 2024-02-17T21:15:47Z Stefanova, Natalia A. Maksimova, Kseniya Yi Rudnitskaya, Ekaterina A. Muraleva, Natalia A. Kolosova, Nataliya G. http://hdl.handle.net/20.500.12701/1739 2022-03-28T09:06:40Z 2018-02-09T00:00:00Z

Название: Association of cerebrovascular dysfunction with the development of Alzheimer’s disease-like pathology in OXYS rats Авторы: Stefanova, Natalia A.; Maksimova, Kseniya Yi; Rudnitskaya, Ekaterina A.; Muraleva, Natalia A.; Kolosova, Nataliya G. Краткий осмотр (реферат): Cerebrovascular dysfunction plays a critical role in the pathogenesis of Alzheimer’s disease (AD): the most common cause of dementia in the elderly. The involvement of neurovasculature disorders in the progression of AD is now increasingly appreciated, but whether they represent initial factors or late-stage pathological changes during the disease is unclear. Using senescence-accelerated OXYS rats, which simulate key characteristics of sporadic AD, we evaluated contributions of cerebrovascular alterations to the disease development. At preclinical, early, and advanced stages of AD-like pathology, in the hippocampus of OXYS and Wistar (control) rats, we evaluated (i) the blood vessel state by histological and electron-microscopic analyses; (ii) differences in gene expression according to RNA sequencing (RNA-Seq) to identify the metabolic processes and pathways associated with blood vessel function; (iii) the amount of vascular endothelial growth factor (VEGF) by western blot and immunohistochemical analysis.

2018-02-09T00:00:00Z Smirnova, Liudmila Seregin, Alexander Boksha, Irina Dmitrieva, Elena Simutkin, German Kornetova, Elena Savushkina, Olga Letova, Anastasia Bokhan, Nikolay Ivanova, Svetlana Zgoda, Victor http://hdl.handle.net/20.500.12701/1689 2022-03-18T04:30:03Z 2019-07-11T00:00:00Z

Название: The difference in serum proteomes in schizophrenia and bipolar disorder Авторы: Smirnova, Liudmila; Seregin, Alexander; Boksha, Irina; Dmitrieva, Elena; Simutkin, German; Kornetova, Elena; Savushkina, Olga; Letova, Anastasia; Bokhan, Nikolay; Ivanova, Svetlana; Zgoda, Victor Краткий осмотр (реферат): Background Purpose of study is revealing significant differences in serum proteomes in schizophrenia and bipolar disorder (BD). Results Quantitative mass-spectrometry based proteomic analysis was used to quantify proteins in the blood serum samples after the depletion of six major blood proteins. Comparison of proteome profiles of different groups revealed 27 proteins being specific for schizophrenia, and 18 – for BD. Protein set in schizophrenia was mostly associated with immune response, cell communication, cell growth and maintenance, protein metabolism and regulation of nucleic acid metabolism. Protein set in BD was mostly associated with immune response, regulating transport processes across cell membrane and cell communication, development of neurons and oligodendrocytes and cell growth. Concentrations of ankyrin repeat domain-containing protein 12 (ANKRD12) and cadherin 5 in serum samples were determined by ELISA. Significant difference between three groups was revealed in ANKRD12 concentration (p = 0.02), with maximum elevation of ANKRD12 concentration (median level) in schizophrenia followed by BD. Cadherin 5 concentration differed significantly (p = 0.035) between schizophrenic patients with prevailing positive symptoms (4.78 [2.71, 7.12] ng/ml) and those with prevailing negative symptoms (1.86 [0.001, 4.11] ng/ml). Conclusions Our results are presumably useful for discovering the new pathways involved in endogenous psychotic disorders.

2019-07-11T00:00:00Z