DSpace Собрание: Frontiers in Physics publishes rigorously peer-reviewed research across the entire field, from experimental, to computational and theoretical physicsFrontiers in Physics publishes rigorously peer-reviewed research across the entire field, from experimental, to computational and theoretical physicshttp://hdl.handle.net/20.500.12701/16352024-02-16T01:59:41Z2024-02-16T01:59:41ZLabel-Free Non-linear Multimodal Optical Microscopy—Basics, Development, and ApplicationsMazumder, NirmalBalla, Naveen K.Zhuo, Guan-YuKistenev, Yury V.Kumar, RajeshKao, Fu-JenBrasselet, SophieNikolaev, Viktor V.Krivova, Natalya A.http://hdl.handle.net/20.500.12701/16362022-03-03T04:44:51Z2019-10-31T00:00:00ZНазвание: Label-Free Non-linear Multimodal Optical Microscopy—Basics, Development, and Applications
Авторы: Mazumder, Nirmal; Balla, Naveen K.; Zhuo, Guan-Yu; Kistenev, Yury V.; Kumar, Rajesh; Kao, Fu-Jen; Brasselet, Sophie; Nikolaev, Viktor V.; Krivova, Natalya A.
Краткий осмотр (реферат): Non-linear optical (NLO) microscopy has proven to be a powerful tool especially
for tissue imaging with sub-cellular resolution, high penetration depth, endogenous
contrast specificity, pinhole-less optical sectioning capability. In this review, we discuss
label-free non-linear optical microscopes including the two-photon fluorescence (TPF),
fluorescence lifetime imaging microscopy (FLIM), polarization-resolved second harmonic
generation (SHG) and coherent anti-Stokes Raman scattering (CARS) techniques with
various samples. The non-linear signals are generated from collagen in tissue (SHG),
amylopectin from starch granules (SHG), sarcomere structure of fresh muscle (SHG),
elastin in skin (TPF), nicotinamide adenine dinucleotide (NADH) in cells (TPF), and
lipid droplets in cells (CARS). Again, the non-linear signals are very specific to the
molecular structure of the sample and its relative orientation to the polarization of
the incident light. Thus, polarization-resolved non-linear optical microscopy provides
high image contrast and quantitative estimate of sample orientation. An overview of
the advancements on polarization-resolved SHG microscopy including Stokes vector
based polarimetry, circular dichroism, and susceptibility are also presented in this review
article. The working principles and corresponding implements of above-mentioned
microscopy techniques are elucidated. The potential of time-resolved TPF lifetime
imaging microscopy (TP-FLIM) is explored by imaging endogenous fluorescence of
NAD(P)H, a key coenzyme in cellular metabolic processes. We also discuss single
laser source time-resolved multimodal CARS-FLIM microscopy using time-correlated
single-photon counting (TCSPC) in combination with continuum generation from
photonic crystal fiber (PCF). Using examples, we demonstrate that the multimodal NLO
microscopy is a powerful tool to assess the molecular specificity with high resolution.2019-10-31T00:00:00Z